Journal: iScience

Article Title: Targeting mutant NPM1-expressing AML with T cell receptor-like antibodies

doi: 10.1016/j.isci.2026.117630

Figure Lengend Snippet:

Article Snippet: Sensor Chip Protein G , Cytiva & GE , Cat# 29179315.

Techniques: Cell-Signaling, Purification, Recombinant, Sequencing, Control, Plasmid Preparation, Extraction, Transfection, Bicinchoninic Acid Protein Assay, Conjugation Assay, Staining, Enzyme-linked Immunosorbent Assay, Software

Journal: bioRxiv

Article Title: Patronin condensation integrates recognition, protection and organization of microtubule minus ends

doi: 10.64898/2026.10.03.756418

Figure Lengend Snippet: (A, B) Representative images (A) and live imaging (B) of S2 cells co-expressing Ena and Patronin. Yellow and blue arrows, condensates before and during fusion; white arrows, condensates after fusion. Scale bar, 1 μm. (C, D) Disruption of phase separation in Patronin-YFW23S disrupts colocalization of Ena and Patronin, whereas introducing HNRNPA1 (Patronin-HNRNPA1) restores their colocalization. Scale bar, 1 μm. (E-G) Images of ubi -promoter driven Patronin and Patronin-YFW23S in stage 9 egg chambers at different focal planes (mid and cortex) of the oocyte (E). Red arrows point to the anterior region of the oocyte. Right, schematic of Patronin and Patronin-YFW23S distribution in the oocyte. Scale bar, 10 μm. (F) Patronin and Patronin-YFW23S fluorescence intensity along the yellow dashed line in the mid oocyte in (E). 0 at x-axis indicates the left end of the dashed line. (G) Quantification of Patronin and Patronin-YFW23S fluorescence intensity at mid oocyte focal plane, n = 4. (H, I) Representative images (H) and their quantification (I) of distribution patterns of ubi -promoter driven Patronin-mNG or Patronin-YFW23S-mNG together with endogenous Shot. Shot was labeled with an anti-Shot antibody. Scale bar, 20 μm. Fluorescence intensity in (I) was measured along the yellow dashed lines in (H). (J-L) (J) Distribution patterns of Patronin-mNG or Patronin-YFW23S-mNG in stage 9 follicle cells. Patronin-mNG is localized in the apical region of follicle cells. Bottom, schematic of Patronin and Patronin-YFW23S distribution in follicle cells. Scale bar, 20 μm. (K-L) Quantification of Patronin and Patronin-YFW23S fluorescence intensity along the yellow dashed line in (J). 0.00 at the x-axis (K) indicates the most apical position. (M, N) Distribution patterns (M) and Quantification (N) of Patronin-mNG or Patronin-YFW23S-mNG in region 3 of germarium, where germline cyst and follicle cells are initially derived. Right, schematic of Patronin and Patronin-YFW23S distribution in the region 3 of germarium. Scale bar, 10 μm. (O, P) Distribution patterns (O) and Quantification (P) of Patronin-mNG or Patronin-YFW23S-mNG together with Shot in region 3 of germarium. Right (O), schematic of Patronin and Patronin-YFW23S distribution with Shot. Scale bar, 5 μm. (Q) Schematic of CRISPR–Cas9-mediated genome engineering to generate endogenous Patronin PrLD mutations in Drosophila . Yellow, sgRNA–Cas9 complexes. (R) Schematic of Drosophila genetic experiments. Notably, the Patronin-YFW23S mutant is homozygous lethal.

Article Snippet: The Patronin-YFW23S knock-in strain was generated by injecting w1118 embryos with in vitro -transcribed sgRNAs (Promega, P1320), a donor plasmid and Cas9 protein (NEB, M0386).

Techniques: Imaging, Expressing, Disruption, Fluorescence, Labeling, Derivative Assay, CRISPR, Drosophila, Mutagenesis